FASTA to CSV

Universal Format Converter

Convert between CSV, TSV, JSON, and Excel. Supports multiple files, primary data, and batch operations.

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AboutFASTA to CSV

Convert FASTA sequence files into CSV rows with ID, description and sequence columns for spreadsheet review or downstream analysis.Category: Scientific Formats

Reviewing sequence headers in bulkFeeding sequence metadata into spreadsheets

The FASTA to CSV tool is compatible with:Genomics PipelinesNCBI Exports.Pull raw sequence records into CSV here, then cross-check specific reads against FASTQ_TO_CSV quality scores or compare against variant calls from VCF_TO_CSV when the same samples went through variant calling.
FASTA is the plain-text standard for representing nucleotide or protein sequences across genomics and bioinformatics — a header line starting with ">" followed by one or more lines of raw sequence data — but that layout is awkward the moment you want to sort, filter, or eyeball hundreds of records at once. This converter splits the file on each ">" marker, separates the sequence ID from its free-text description, and joins the wrapped sequence lines back into a single continuous string per record. The output is one row per sequence with ID, description and full sequence as separate columns, which is far easier to scan, filter by length, or merge with metadata in a spreadsheet than the raw multi-line format. It handles multi-record FASTA files of any size in-browser, so you're not limited by what a command-line tool or institutional pipeline happens to have installed.

Frequently Asked Questions:

What does each output row represent?

One sequence record, with its ID, description, and full joined sequence as separate columns.

Does it work with multi-record FASTA files?

Yes, it splits the file on every ">" header, however many records it contains.

Built for researchers who want to sort, filter or skim hundreds of sequence records without opening a bioinformatics toolkit.